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Incorporation of sulfide ions into the cadmium(II)-thiolate cluster of Cicer arietinum metallothionein2


Wan, Xiaoqiong; Freisinger, Eva (2013). Incorporation of sulfide ions into the cadmium(II)-thiolate cluster of Cicer arietinum metallothionein2. Inorganic Chemistry, 52(2):785-792.

Abstract

The plant metallothionein2 from <i>Cicer arietinum</i> (chickpea), cic-MT2, is known to coordinate five divalent metal ions such as ZnII or CdII, which are arranged in a single metal thiolate cluster. When the ZnII form of the protein is titrated with CdII ions in the presence of sulfide ions, an increased CdII binding capacity and concomitant incorporation of sulfide ions into the cluster are observed. The exact stoichiometry of this novel cluster, its spectroscopic properties, and the significantly increased pH stability are analyzed with different techniques, including UV and circular dichroism spectroscopy and calorimetric assays. Limited proteolytic digestion provides information about the spacial arrange- ment of the cluster within the protein. Increasing the CdII scavenging properties of a metallothionein by additionally recruiting sulfide ions might be an economic and very efficient detoxification strategy for plants.

Abstract

The plant metallothionein2 from <i>Cicer arietinum</i> (chickpea), cic-MT2, is known to coordinate five divalent metal ions such as ZnII or CdII, which are arranged in a single metal thiolate cluster. When the ZnII form of the protein is titrated with CdII ions in the presence of sulfide ions, an increased CdII binding capacity and concomitant incorporation of sulfide ions into the cluster are observed. The exact stoichiometry of this novel cluster, its spectroscopic properties, and the significantly increased pH stability are analyzed with different techniques, including UV and circular dichroism spectroscopy and calorimetric assays. Limited proteolytic digestion provides information about the spacial arrange- ment of the cluster within the protein. Increasing the CdII scavenging properties of a metallothionein by additionally recruiting sulfide ions might be an economic and very efficient detoxification strategy for plants.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Language:English
Date:2013
Deposited On:28 Mar 2013 11:46
Last Modified:05 Apr 2016 16:40
Publisher:American Chemical Society
ISSN:0020-1669
Additional Information:This document is the Accepted Manuscript version of a Published Work that appeared in final form in Inorganic chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/ic301907j.
Publisher DOI:https://doi.org/10.1021/ic301907j

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