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Optimization of 14C liquid scintillation counting of plant and soil lipids to trace short term formation, translocation and degradation of lipids


Wiesenberg, Guido L B; Gocke, Martina; Kuzyakov, Yakov (2010). Optimization of 14C liquid scintillation counting of plant and soil lipids to trace short term formation, translocation and degradation of lipids. Journal of Radioanalytical and Nuclear Chemistry, 284(1):99-108.

Abstract

Two powerful approaches are frequently used to trace incorporation and degradation of plant derived C in soil: 14C labelling/chasing and analysis of lipid composition. In this study, we coupled these approaches in order to trace short term incorporation of plant derived lipids into rhizosphere and non-rhizosphere soil. Methodological optimization was required and implied 14C liquid scintillation counting improvement for plant lipid extracts taking into account organic solvents, solvent-to-scintillation cocktail ratio, and amount of lipids. Following method optimization, 14C data of fatty acids indicated a notable contribution of root derived lipids to rhizosphere and non- rhizosphere soil. Coupling of 14C labelling/chasing with lipid analysis is a powerful and cheap approach for tracing of root derived C in soil allowing for estimation of C budget, for determination of C formation and translocation within plants and from plant to soil, as well as for identi- fication of short term dynamics of specific compound classes within soil.

Abstract

Two powerful approaches are frequently used to trace incorporation and degradation of plant derived C in soil: 14C labelling/chasing and analysis of lipid composition. In this study, we coupled these approaches in order to trace short term incorporation of plant derived lipids into rhizosphere and non-rhizosphere soil. Methodological optimization was required and implied 14C liquid scintillation counting improvement for plant lipid extracts taking into account organic solvents, solvent-to-scintillation cocktail ratio, and amount of lipids. Following method optimization, 14C data of fatty acids indicated a notable contribution of root derived lipids to rhizosphere and non- rhizosphere soil. Coupling of 14C labelling/chasing with lipid analysis is a powerful and cheap approach for tracing of root derived C in soil allowing for estimation of C budget, for determination of C formation and translocation within plants and from plant to soil, as well as for identi- fication of short term dynamics of specific compound classes within soil.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Geography
Dewey Decimal Classification:910 Geography & travel
Language:English
Date:2010
Deposited On:14 Mar 2013 10:21
Last Modified:05 Apr 2016 16:41
Publisher:Springer
ISSN:0236-5731
Publisher DOI:https://doi.org/10.1007/s10967-010-0450-7

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