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Vignetting and photo-bleaching correction in automated fluorescence microscopy from an array of overlapping images


Piccinini, Filippo; Bevilacqua, Alessandro; Smith, Kevin; Horvath, Peter (2013). Vignetting and photo-bleaching correction in automated fluorescence microscopy from an array of overlapping images. In: 10th IEEE International Symposium on Biomedical Imaging (ISBI), San Francisco, CA, U.S.A., 2013 - 2013.

Abstract

We propose a novel acquisition scheme and non-parametric multi-image based method for correcting illumination in fluorescence images. Our approach measures changes in intensity of the subject by moving the microscope stage at regularly spaced intervals, and exploits this information to learn the correction function. The acquisition process and learning are performed prior to imaging, and take only a few minutes. Afterwards, images can be corrected for vignetting and photo-bleaching effects on the fly. Our approach can be implemented in any microscope with a motorized stage, and does not require a reference calibration slide. Experiments demonstrate that our method outperforms standard approaches to illumination correction.

Abstract

We propose a novel acquisition scheme and non-parametric multi-image based method for correcting illumination in fluorescence images. Our approach measures changes in intensity of the subject by moving the microscope stage at regularly spaced intervals, and exploits this information to learn the correction function. The acquisition process and learning are performed prior to imaging, and take only a few minutes. Afterwards, images can be corrected for vignetting and photo-bleaching effects on the fly. Our approach can be implemented in any microscope with a motorized stage, and does not require a reference calibration slide. Experiments demonstrate that our method outperforms standard approaches to illumination correction.

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4 citations in Web of Science®
6 citations in Scopus®
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43 downloads since deposited on 17 Apr 2013
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Additional indexing

Item Type:Conference or Workshop Item (Paper), refereed, original work
Communities & Collections:Special Collections > SystemsX.ch
Special Collections > SystemsX.ch > Systems Biology IT
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Event End Date:2013
Deposited On:17 Apr 2013 10:48
Last Modified:07 Dec 2017 20:44

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