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Ionic Liquids as Matrices in MicrofluidicSample Deposition for High-mass Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry


Weidmann, Simon; Kemmerling, Simon; Mädler, Stefanie; Stahlberg, Henning; Braun, Thomas; Zenobi, Renato (2012). Ionic Liquids as Matrices in MicrofluidicSample Deposition for High-mass Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. European Journal of Mass Spectrometry, 18:279-286.

Abstract

Sample preparation for MALDI-MS via a microfluidic deposition device using ionic liquid matrices addresses several problems of standard protocols with crystalline matrices, such as the heterogeneity of sample spots due to the co-crystallization of sample and matrix, and the limited capability for high-throughput analysis. Since ionic liquid matrices do not solidify during the measurement, the resulting sample spots are homogeneous. The use of these matrices is also beneficial for automated sample preparation, since crystallization of the matrix is avoided, and thus no clogging of the spotting device can occur. The applicability of ionic liquids to the analysis of biomolecules with high molecular weights, up to ˜ 1 MDa is shown, as well as a good sensitivity (5 fmol) for recombinant human fibronectin, a protein with a molecular weight of 226 kDa. Microfluidic sample deposition of proteins with high molecular weights will in the future allow parallel sample preparation for MALDI-MS and for electron microscopy.

Abstract

Sample preparation for MALDI-MS via a microfluidic deposition device using ionic liquid matrices addresses several problems of standard protocols with crystalline matrices, such as the heterogeneity of sample spots due to the co-crystallization of sample and matrix, and the limited capability for high-throughput analysis. Since ionic liquid matrices do not solidify during the measurement, the resulting sample spots are homogeneous. The use of these matrices is also beneficial for automated sample preparation, since crystallization of the matrix is avoided, and thus no clogging of the spotting device can occur. The applicability of ionic liquids to the analysis of biomolecules with high molecular weights, up to ˜ 1 MDa is shown, as well as a good sensitivity (5 fmol) for recombinant human fibronectin, a protein with a molecular weight of 226 kDa. Microfluidic sample deposition of proteins with high molecular weights will in the future allow parallel sample preparation for MALDI-MS and for electron microscopy.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:Special Collections > SystemsX.ch
Special Collections > SystemsX.ch > Research, Technology and Development Projects > CINA
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:July 2012
Deposited On:24 Jun 2013 08:57
Last Modified:17 Feb 2018 01:45
Publisher:I M Publications
ISSN:1469-0667
OA Status:Green
Publisher DOI:https://doi.org/10.1255/ejms.1182

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