Header

UZH-Logo

Maintenance Infos

Ionic Liquids as Matrices in MicrofluidicSample Deposition for High-mass Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry


Weidmann, Simon; Kemmerling, Simon; Mädler, Stefanie; Stahlberg, Henning; Braun, Thomas; Zenobi, Renato (2012). Ionic Liquids as Matrices in MicrofluidicSample Deposition for High-mass Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. European Journal of Mass Spectrometry, 18:279-286.

Abstract

Sample preparation for MALDI-MS via a microfluidic deposition device using ionic liquid matrices addresses several problems of standard protocols with crystalline matrices, such as the heterogeneity of sample spots due to the co-crystallization of sample and matrix, and the limited capability for high-throughput analysis. Since ionic liquid matrices do not solidify during the measurement, the resulting sample spots are homogeneous. The use of these matrices is also beneficial for automated sample preparation, since crystallization of the matrix is avoided, and thus no clogging of the spotting device can occur. The applicability of ionic liquids to the analysis of biomolecules with high molecular weights, up to ˜ 1 MDa is shown, as well as a good sensitivity (5 fmol) for recombinant human fibronectin, a protein with a molecular weight of 226 kDa. Microfluidic sample deposition of proteins with high molecular weights will in the future allow parallel sample preparation for MALDI-MS and for electron microscopy.

Abstract

Sample preparation for MALDI-MS via a microfluidic deposition device using ionic liquid matrices addresses several problems of standard protocols with crystalline matrices, such as the heterogeneity of sample spots due to the co-crystallization of sample and matrix, and the limited capability for high-throughput analysis. Since ionic liquid matrices do not solidify during the measurement, the resulting sample spots are homogeneous. The use of these matrices is also beneficial for automated sample preparation, since crystallization of the matrix is avoided, and thus no clogging of the spotting device can occur. The applicability of ionic liquids to the analysis of biomolecules with high molecular weights, up to ˜ 1 MDa is shown, as well as a good sensitivity (5 fmol) for recombinant human fibronectin, a protein with a molecular weight of 226 kDa. Microfluidic sample deposition of proteins with high molecular weights will in the future allow parallel sample preparation for MALDI-MS and for electron microscopy.

Statistics

Citations

8 citations in Web of Science®
6 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

2195 downloads since deposited on 24 Jun 2013
19 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:Special Collections > SystemsX.ch
Special Collections > SystemsX.ch > Research, Technology and Development Projects > CINA
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:July 2012
Deposited On:24 Jun 2013 08:57
Last Modified:26 Jan 2017 08:54
Publisher:I M Publications
ISSN:1469-0667
Publisher DOI:https://doi.org/10.1255/ejms.1182

Download

Preview Icon on Download
Preview
Filetype: PDF
Size: 1MB
View at publisher

TrendTerms

TrendTerms displays relevant terms of the abstract of this publication and related documents on a map. The terms and their relations were extracted from ZORA using word statistics. Their timelines are taken from ZORA as well. The bubble size of a term is proportional to the number of documents where the term occurs. Red, orange, yellow and green colors are used for terms that occur in the current document; red indicates high interlinkedness of a term with other terms, orange, yellow and green decreasing interlinkedness. Blue is used for terms that have a relation with the terms in this document, but occur in other documents.
You can navigate and zoom the map. Mouse-hovering a term displays its timeline, clicking it yields the associated documents.

Author Collaborations