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Kinetic subpopulations detected by single-molecule spectroscopy: Fundamental property of functional nucleic acids or experimental artefact?


König, Sebastian L B; Kowerko, Danny; Sigel, Roland K O (2013). Kinetic subpopulations detected by single-molecule spectroscopy: Fundamental property of functional nucleic acids or experimental artefact? CHIMIA International Journal for Chemistry, 67(4):240-243.

Abstract

Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1* interaction, two RNA sequences that play an important role in group II intron mediated self-cleavage. Further examples of dynamic heterogeneity in functional nucleic acids are provided and the possible origins of this phenomenon are discussed.

Abstract

Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1* interaction, two RNA sequences that play an important role in group II intron mediated self-cleavage. Further examples of dynamic heterogeneity in functional nucleic acids are provided and the possible origins of this phenomenon are discussed.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Language:English
Date:4 April 2013
Deposited On:08 Aug 2013 12:11
Last Modified:18 May 2017 08:02
Publisher:Swiss Chemical Society
ISSN:0009-4293
Additional Information:Copyright © Swiss Chemical Society: CHIMIA, Vol. 67(4): 240-243, 2013
Publisher DOI:https://doi.org/10.2533/chimia.2013.240

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