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In vitro Transcription and Purification of RNAs of Different Size


Gallo, S; Furler, M; Sigel, Roland K O (2005). In vitro Transcription and Purification of RNAs of Different Size. CHIMIA International Journal for Chemistry, 59(11):812-816.

Abstract

In vitro run-off transcription from a double-stranded DNA template by T7 RNA polymerase is an elegant way to obtain highly pure and uniform RNA oligonucleotides of lengths ranging from about 15 to several thousand nucleotides. Here we describe the different strategies applied and optimized in our laboratory to enzymatically synthesize RNAs as necessary when working at the interface of bioinorganic chemistry, coordination chemistry, RNA biochemistry and structural biology.

Abstract

In vitro run-off transcription from a double-stranded DNA template by T7 RNA polymerase is an elegant way to obtain highly pure and uniform RNA oligonucleotides of lengths ranging from about 15 to several thousand nucleotides. Here we describe the different strategies applied and optimized in our laboratory to enzymatically synthesize RNAs as necessary when working at the interface of bioinorganic chemistry, coordination chemistry, RNA biochemistry and structural biology.

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Additional indexing

Item Type:Journal Article, refereed, further contribution
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Language:English
Date:November 2005
Deposited On:08 Aug 2013 07:47
Last Modified:18 May 2017 08:03
Publisher:Swiss Chemical Society
ISSN:0009-4293
Publisher DOI:https://doi.org/10.2533/000942905777675589

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