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Phylogenetic analysis and expression of zebrafish transient receptor potential melastatin family genes


Kastenhuber, Edda; Gesemann, Matthias; Mickoleit, Michaela; Neuhauss, Stephan C F (2013). Phylogenetic analysis and expression of zebrafish transient receptor potential melastatin family genes. Developmental Dynamics, 242(11):1236-1249.

Abstract

Background: The transient receptor potential melastatin (TRPM) gene family belongs to the superfamily of non-selective TRP ion channels. TRP channels are cellular sensors, detecting a multitude of inputs, including temperature, light, chemical and mechanical stimuli. Recent studies revealed diverse roles during development, linking TRP channels to differentiation, proliferation, cell motility, cell death and survival. A detailed description of this gene family in the zebrafish is still missing. Results: Phylogenetic analysis revealed 11 trpm genes in the zebrafish genome. The zebrafish orthologs of mammalian TRPM1 and TRPM4 are duplicated and quadruplicated, respectively, and TRPM8, a cold sensitive channel has been lost in zebrafish. Whole-mount in situ hybridization experiments revealed dynamic expression pattern of trpm genes in the developing embryo and early larva. Transcripts were mainly found in neural cell clusters, but also in tissues involved in ion homeostasis. Conclusions: Our results suggest a role of TRPM channels in sensory information processing, including vision, olfaction, taste, and mechanosensation. An involvement in developmental processes is likely, as some trpm genes were found to be expressed in differentiating cells. Our data now provides a basis for functional analyses of this gene family of ion channels in the vertebrate model organism Danio rerio.

Abstract

Background: The transient receptor potential melastatin (TRPM) gene family belongs to the superfamily of non-selective TRP ion channels. TRP channels are cellular sensors, detecting a multitude of inputs, including temperature, light, chemical and mechanical stimuli. Recent studies revealed diverse roles during development, linking TRP channels to differentiation, proliferation, cell motility, cell death and survival. A detailed description of this gene family in the zebrafish is still missing. Results: Phylogenetic analysis revealed 11 trpm genes in the zebrafish genome. The zebrafish orthologs of mammalian TRPM1 and TRPM4 are duplicated and quadruplicated, respectively, and TRPM8, a cold sensitive channel has been lost in zebrafish. Whole-mount in situ hybridization experiments revealed dynamic expression pattern of trpm genes in the developing embryo and early larva. Transcripts were mainly found in neural cell clusters, but also in tissues involved in ion homeostasis. Conclusions: Our results suggest a role of TRPM channels in sensory information processing, including vision, olfaction, taste, and mechanosensation. An involvement in developmental processes is likely, as some trpm genes were found to be expressed in differentiating cells. Our data now provides a basis for functional analyses of this gene family of ion channels in the vertebrate model organism Danio rerio.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2013
Deposited On:12 Sep 2013 15:41
Last Modified:05 Apr 2016 16:58
Publisher:Wiley-Blackwell
ISSN:1058-8388
Publisher DOI:https://doi.org/10.1002/dvdy.24020
PubMed ID:23908157

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