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In vitro evaluation of the oxidation efficacy of transgingival photodynamic therapy


Ronay, Valerie; Buchalla, Wolfgang; Sahrmann, Philipp; Attin, Thomas; Schmidlin, Patrick R (2013). In vitro evaluation of the oxidation efficacy of transgingival photodynamic therapy. Acta Odontologica Scandinavica, 71(5):1216-1220.

Abstract

OBJECTIVE: To evaluate the capability of soft laser light to penetrate blood, serum, gingival connective tissue and pure collagen type I.
MATERIALS AND METHODS: A 1:1 mixture of methylene blue (MB) and diphenylisobenzofuran (DPBF) was irradiated for 60 s with a diode laser (670 nm, 0.3 W) through blood, serum, gingival connective tissue and collagen type I (2 mm transillumination thickness). The oxidation of DPBF by MB was determined spectrophotometrically by measuring the optical density (oD) at 410 nm. The absorption spectra of DPBF/MB irradiated through MB (1%) and strawberry red solution (3%) served as control.
RESULTS: The mean oD of non-irradiated DPBF/MB was 1.98 ± 0.04. Irradiation through MB showed no oxidation of DPBF (1.98 ± 0.02; p > 0.05), while interposition of strawberry red and serum resulted in almost complete oxidation of DPBF (0.13 ± 0.09, 0.06 ± 0.03; p ≤ 0.0001). Irradiation through gingiva and collagen reduced the oxidation of DPBF significantly (1.0 ± 0.04, 0.7 ± 0.04; p ≤ 0.0001), accounting for 50% to 35% of the non-irradiated DPBF/MB solution.
CONCLUSION: Red light from a diode laser can penetrate blood and gingival tissues. However, light absorption for collagen and connective tissue can hamper the oxidation process.

Abstract

OBJECTIVE: To evaluate the capability of soft laser light to penetrate blood, serum, gingival connective tissue and pure collagen type I.
MATERIALS AND METHODS: A 1:1 mixture of methylene blue (MB) and diphenylisobenzofuran (DPBF) was irradiated for 60 s with a diode laser (670 nm, 0.3 W) through blood, serum, gingival connective tissue and collagen type I (2 mm transillumination thickness). The oxidation of DPBF by MB was determined spectrophotometrically by measuring the optical density (oD) at 410 nm. The absorption spectra of DPBF/MB irradiated through MB (1%) and strawberry red solution (3%) served as control.
RESULTS: The mean oD of non-irradiated DPBF/MB was 1.98 ± 0.04. Irradiation through MB showed no oxidation of DPBF (1.98 ± 0.02; p > 0.05), while interposition of strawberry red and serum resulted in almost complete oxidation of DPBF (0.13 ± 0.09, 0.06 ± 0.03; p ≤ 0.0001). Irradiation through gingiva and collagen reduced the oxidation of DPBF significantly (1.0 ± 0.04, 0.7 ± 0.04; p ≤ 0.0001), accounting for 50% to 35% of the non-irradiated DPBF/MB solution.
CONCLUSION: Red light from a diode laser can penetrate blood and gingival tissues. However, light absorption for collagen and connective tissue can hamper the oxidation process.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic for Preventive Dentistry, Periodontology and Cariology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2013
Deposited On:18 Dec 2013 08:18
Last Modified:05 Apr 2016 17:16
Publisher:Informa Healthcare
ISSN:0001-6357
Publisher DOI:https://doi.org/10.3109/00016357.2012.757639
PubMed ID:23320555

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