Header

UZH-Logo

Maintenance Infos

Correspondence of high-frequency ultrasound and histomorphometry of healing rabbit achilles tendon tissue


Buschmann, Johanna; Puippe, Gilbert; Meier Bürgisser, Gabriella; Bonavoglia, Eliana; Giovanoli, Pietro; Calcagni, Maurizio (2014). Correspondence of high-frequency ultrasound and histomorphometry of healing rabbit achilles tendon tissue. Connective Tissue Research, 55(2):123-131.

Abstract

Abstract Objectives: Static and dynamic high-frequency ultrasound of healing rabbit Achilles tendons were set in relationship to histomorphometric analyses at three and six weeks post-surgery. Materials and Methods: Twelve New Zealand White rabbits received a clean-cut Achilles tendon laceration (the medial and lateral M. gastrocnemius) and were repaired with a 4-strand Becker suture. Six rabbits got additionally a tight polyester urethane tube at the repair site in order to vary the adhesion extent. Tendons were analyzed by static and dynamic ultrasound (control: healthy contralateral legs). The ultrasound outcome was corresponded to the tendon shape, tenocyte and tenoblast density, tenocyte and tenoblast nuclei width, collagen fibre orientation and adhesion extent. Results: The spindle-like morphology of healing tendons (ultrasound) was confirmed by the swollen epitenon (histology). Prediction of adhesion formation by dynamic ultrasound assessment was confirmed by histology (contact region to surrounding tissue). Hyperechogenic areas corresponded to acellular zones with aligned fibres and hypoechogenic zones to not yet oriented fibres and to cell rich areas. Conclusions: These findings add new in-depth structural knowledge to the established non-invasive analytical tool, ultrasound.

Abstract

Abstract Objectives: Static and dynamic high-frequency ultrasound of healing rabbit Achilles tendons were set in relationship to histomorphometric analyses at three and six weeks post-surgery. Materials and Methods: Twelve New Zealand White rabbits received a clean-cut Achilles tendon laceration (the medial and lateral M. gastrocnemius) and were repaired with a 4-strand Becker suture. Six rabbits got additionally a tight polyester urethane tube at the repair site in order to vary the adhesion extent. Tendons were analyzed by static and dynamic ultrasound (control: healthy contralateral legs). The ultrasound outcome was corresponded to the tendon shape, tenocyte and tenoblast density, tenocyte and tenoblast nuclei width, collagen fibre orientation and adhesion extent. Results: The spindle-like morphology of healing tendons (ultrasound) was confirmed by the swollen epitenon (histology). Prediction of adhesion formation by dynamic ultrasound assessment was confirmed by histology (contact region to surrounding tissue). Hyperechogenic areas corresponded to acellular zones with aligned fibres and hypoechogenic zones to not yet oriented fibres and to cell rich areas. Conclusions: These findings add new in-depth structural knowledge to the established non-invasive analytical tool, ultrasound.

Statistics

Citations

2 citations in Web of Science®
3 citations in Scopus®
Google Scholar™

Altmetrics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
04 Faculty of Medicine > University Hospital Zurich > Clinic for Thoracic Surgery
04 Faculty of Medicine > University Hospital Zurich > Clinic for Reconstructive Surgery
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2014
Deposited On:20 Jan 2014 10:35
Last Modified:05 Apr 2016 17:23
Publisher:Informa Healthcare
ISSN:0300-8207
Publisher DOI:https://doi.org/10.3109/03008207.2013.870162
PubMed ID:24283274

Download

Full text not available from this repository.
View at publisher