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Examining female meiocytes of maize by confocal microscopy


Barrell, Philippa; Grossniklaus, Ueli (2013). Examining female meiocytes of maize by confocal microscopy. In: Pawlowski, Wojciech. Plant Meiosis: Methods and Protocols , Methods in Molecular Biology. Springer, 45-52.

Abstract

Meiosis is a key step in sexual plant reproduction. Cytological analysis of meiosis enables hypotheses of gene function during meiosis to be confirmed or discarded. Observation of meiosis in the female reproductive organs in many higher plants is technically challenging because the cells are enclosed deep within the nucellus and ovule tissues. In this chapter, we describe a simple staining procedure using Schiff's reagent optimized for use with confocal microscopy to observe meiosis within intact ovules of Zea mays (maize). Using this procedure we present images taken by confocal laser scanning microscopy of wild-type meiotic cells in whole-mount maize ovules.

Abstract

Meiosis is a key step in sexual plant reproduction. Cytological analysis of meiosis enables hypotheses of gene function during meiosis to be confirmed or discarded. Observation of meiosis in the female reproductive organs in many higher plants is technically challenging because the cells are enclosed deep within the nucellus and ovule tissues. In this chapter, we describe a simple staining procedure using Schiff's reagent optimized for use with confocal microscopy to observe meiosis within intact ovules of Zea mays (maize). Using this procedure we present images taken by confocal laser scanning microscopy of wild-type meiotic cells in whole-mount maize ovules.

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Item Type:Book Section, refereed, further contribution
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Date:2013
Deposited On:22 Jan 2014 13:41
Last Modified:16 Feb 2018 19:14
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:990
ISSN:1064-3745
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-62703-333-6_5
PubMed ID:23559201

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