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The expression of mucin genes and the presence of mucin gene products in the equine endometrium


Maischberger, E; Cummins, C A; Fitzpatrick, E; Gallagher, M E; Worrall, S; Rousseau, K; Thornton, D J; Meijer, W G; Miranda-CasoLuengo, R; Duggan, V E; Carrington, S D; Irwin, J A; Reid, C J (2013). The expression of mucin genes and the presence of mucin gene products in the equine endometrium. Research in Veterinary Science, 95:169-175.

Abstract

In the equine reproductive tract, little is known about mucin gene expression and the role of mucins in barrier function and host-cell interaction. The aims of the study were to identify equine orthologs of mammalian mucin genes using available equine sequence data, to profile expression of equine orthologous mucin genes in the endometrium using reverse transcriptase polymerase chain reaction (RT-PCR), to determine spatial expression patterns of mucin genes using in situ hybridisation, and to confirm the presence of mucin gene products using Western blotting and equine-specific mucin antibodies during oestrus and dioestrus. While the mucin gene expression pattern in equine endometrium is similar to that of other mammals, several mucins appear to be uniquely expressed in this tissue (eqMUC3B, 7, 18, and 20) and one is hormonally regulated (eqMUC3B).

Abstract

In the equine reproductive tract, little is known about mucin gene expression and the role of mucins in barrier function and host-cell interaction. The aims of the study were to identify equine orthologs of mammalian mucin genes using available equine sequence data, to profile expression of equine orthologous mucin genes in the endometrium using reverse transcriptase polymerase chain reaction (RT-PCR), to determine spatial expression patterns of mucin genes using in situ hybridisation, and to confirm the presence of mucin gene products using Western blotting and equine-specific mucin antibodies during oestrus and dioestrus. While the mucin gene expression pattern in equine endometrium is similar to that of other mammals, several mucins appear to be uniquely expressed in this tissue (eqMUC3B, 7, 18, and 20) and one is hormonally regulated (eqMUC3B).

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Equine Department
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Language:English
Date:2013
Deposited On:10 Feb 2014 14:05
Last Modified:08 Dec 2017 03:04
Publisher:Elsevier
ISSN:0034-5288
Publisher DOI:https://doi.org/10.1016/j.rvsc.2013.03.012

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