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Single molecule interactions studied by using a modified DNA sequencer: a comparison with surface plasmon resonance


Sobek, Jens; Schauer, Stefan; Rehrauer, Hubert; Fischer, David; Patrignani, Andrea; Landgraf, Stephan; Schlapbach, Ralph (2013). Single molecule interactions studied by using a modified DNA sequencer: a comparison with surface plasmon resonance. In: SPIE, San Francisco, 2 February 2013 - 2 February 2013, 85960Y.

Abstract

In this study we established the principles for using a commercial off-the-shelf DNA sequencer (RS, Pacific Biosciences) to measure biomolecular interactions.1,2 Binding between single oligonucleotides immobilized at the bottom of a nanowell to a complementary DNA strand was used as a model system. The influence of the labeling dye on the hybridization of a 7mer oligonucleotide was investigated. The resulting association and dissociation rate constants (kon and koff) and the calculated dissociation constants (Kd) were compared to data obtained from surface plasmon resonance (SPR) measurements. In the present study we identified a good agreement of the determined kinetic constants by the two methods investigated.

Abstract

In this study we established the principles for using a commercial off-the-shelf DNA sequencer (RS, Pacific Biosciences) to measure biomolecular interactions.1,2 Binding between single oligonucleotides immobilized at the bottom of a nanowell to a complementary DNA strand was used as a model system. The influence of the labeling dye on the hybridization of a 7mer oligonucleotide was investigated. The resulting association and dissociation rate constants (kon and koff) and the calculated dissociation constants (Kd) were compared to data obtained from surface plasmon resonance (SPR) measurements. In the present study we identified a good agreement of the determined kinetic constants by the two methods investigated.

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Additional indexing

Item Type:Conference or Workshop Item (Paper), refereed, original work
Communities & Collections:04 Faculty of Medicine > Functional Genomics Center Zurich
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Event End Date:2 February 2013
Deposited On:28 Jan 2014 09:33
Last Modified:21 Nov 2017 17:09
Publisher:SPIE - International Society for Optical Engineering
Series Name:Proceedings of SPIE
ISSN:0277-786X
Publisher DOI:https://doi.org/10.1117/12.2002953

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