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Die Rolle von PGE2 als luteotroper Faktor in der frühen Gelbkörperphase der trächtigen und nicht trächtigen Hündin und seine Bedeutung bei der Regulation des Steroidogenic Acute Regulatory (StAR) Proteins


Fox, Barbara. Die Rolle von PGE2 als luteotroper Faktor in der frühen Gelbkörperphase der trächtigen und nicht trächtigen Hündin und seine Bedeutung bei der Regulation des Steroidogenic Acute Regulatory (StAR) Proteins. 2013, University of Zurich, Vetsuisse Faculty.

Abstract

Prostaglandins were described as potent mediators in the auto/paracrine regulation of reproduction. The canine Corpus luteum (Cl) is in part independent of gonadotrop support in the first third of diestrus. Analog to other species, it was postulated, that locally produced prostaglandins act via auto/paracrine mechanisms during this phase. Earlier experiments showed, that both cyclooxygenase 2 (COX2) and prostaglandin E2 (PGE2) -synthase (PGES) are strongly expressed in the early Cl-phase. To support the hypothesis of an auto/paracrine effect of PGE2, canine luteal cells were isolated in the early luteal phase and stimulated with PGE2. An upregulated StAR-promotor activity, an increased StAR-protein expression and an enhanced synthesis of P4 could be shown. In contrast, the expression of cytochrome P450scc and 3! hydroxysteroid dehydrogenase/$-5/$-4 isomerase (3!HSD) remained unaffected. Additionally, the expression of the prostaglandin transporter (PGT) in Cl of pregnant and nonpregnant dogs was assessed and revealed a time-dependent expression with strongest signals in the early luteal phase. Consequently, PGE2 proved to be a steroidogenic factor in luteal cells isolated during the early Cl-phase, while the expression pattern of PGT supports the hypothesis of prostaglandins acting via auto/paracrine mechanisms in this period.

Abstract

Prostaglandins were described as potent mediators in the auto/paracrine regulation of reproduction. The canine Corpus luteum (Cl) is in part independent of gonadotrop support in the first third of diestrus. Analog to other species, it was postulated, that locally produced prostaglandins act via auto/paracrine mechanisms during this phase. Earlier experiments showed, that both cyclooxygenase 2 (COX2) and prostaglandin E2 (PGE2) -synthase (PGES) are strongly expressed in the early Cl-phase. To support the hypothesis of an auto/paracrine effect of PGE2, canine luteal cells were isolated in the early luteal phase and stimulated with PGE2. An upregulated StAR-promotor activity, an increased StAR-protein expression and an enhanced synthesis of P4 could be shown. In contrast, the expression of cytochrome P450scc and 3! hydroxysteroid dehydrogenase/$-5/$-4 isomerase (3!HSD) remained unaffected. Additionally, the expression of the prostaglandin transporter (PGT) in Cl of pregnant and nonpregnant dogs was assessed and revealed a time-dependent expression with strongest signals in the early luteal phase. Consequently, PGE2 proved to be a steroidogenic factor in luteal cells isolated during the early Cl-phase, while the expression pattern of PGT supports the hypothesis of prostaglandins acting via auto/paracrine mechanisms in this period.

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Item Type:Dissertation
Referees:Kowalewski M P, Reichler I M
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Anatomy
Dewey Decimal Classification:570 Life sciences; biology
Language:German
Date:2013
Deposited On:28 Jan 2014 13:51
Last Modified:05 Apr 2016 17:31

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