Header

UZH-Logo

Maintenance Infos

Using plethysmography to determine erythropoietin's impact on neural control of ventilation


Seaborn, Tommy; Gassmann, Max; Soliz, Jorge (2013). Using plethysmography to determine erythropoietin's impact on neural control of ventilation. In: Ghezzi, Pietro; Cerami, Anthony. Tissue-Protective Cytokines: Methods and Protocols, Methods in Molecular Biology. Heidelberg: Springer, 303-314.

Abstract

The evaluation of respiratory parameters often requires the use of anesthetics (that depress the neural -network controlling respiration), and/or ways to restrain the animal's mobility (that produces a stress-dependent increase of respiration). Consequently, the establishment of plethysmography represented an invaluable technique in respiratory physiology. Plethysmography, indeed, allows the assessment of ventilatory parameters on living, unanesthetized, and unrestrained animals. The conception of the barometric plethysmography relies on the fact that an animal placed inside a hermetically closed chamber generates through its breathing a fluctuation of pressure in the chamber than can be recorded. Thus, the respiratory frequency and the tidal volume can be directly measured, while the animal's ventilation is calculated indirectly by the multiplication of these two parameters. In our hands, plethysmography was a key tool to investigate the impact of erythropoietin (Epo) on the neural control of hypoxic ventilation in mice.

Abstract

The evaluation of respiratory parameters often requires the use of anesthetics (that depress the neural -network controlling respiration), and/or ways to restrain the animal's mobility (that produces a stress-dependent increase of respiration). Consequently, the establishment of plethysmography represented an invaluable technique in respiratory physiology. Plethysmography, indeed, allows the assessment of ventilatory parameters on living, unanesthetized, and unrestrained animals. The conception of the barometric plethysmography relies on the fact that an animal placed inside a hermetically closed chamber generates through its breathing a fluctuation of pressure in the chamber than can be recorded. Thus, the respiratory frequency and the tidal volume can be directly measured, while the animal's ventilation is calculated indirectly by the multiplication of these two parameters. In our hands, plethysmography was a key tool to investigate the impact of erythropoietin (Epo) on the neural control of hypoxic ventilation in mice.

Statistics

Citations

Altmetrics

Downloads

1 download since deposited on 28 Jan 2014
0 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Integrative Human Physiology
05 Vetsuisse Faculty > Institute of Veterinary Physiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2013
Deposited On:28 Jan 2014 16:26
Last Modified:05 Apr 2016 17:33
Publisher:Springer
Number:Vol. 982
ISSN:1064-3745
ISBN:978-1-62703-307-7
Publisher DOI:https://doi.org/10.1007/978-1-62703-308-4_18
PubMed ID:23456876

Download