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The fine structure of the dopaminergic innervation of area 10 of macaque prefrontal cortex


Martin, Kevan A C; Spühler, Isabelle A (2013). The fine structure of the dopaminergic innervation of area 10 of macaque prefrontal cortex. European Journal of Neuroscience, 37(7):1061-1071.

Abstract

In common with other areas of the prefrontal cortex, activity in frontopolar area 10 is probably modulated by dopamine. We studied the dopaminergic innervation of monkey prefrontal area 10 by immunostaining with tyrosine hydroxylase (TH) antibodies. TH-positive axons in layer 3 were examined by electron microscopy of series of ultrathin sections. TH-positive boutons containing vesicles were sparse (2 × 10−4 per μm3) and the majority (94%, n = 52) had no identifiable synaptic specialization, which supports the hypothesis that dopamine is released non-synaptically and raises the question of whether the local microenvironment surrounding the boutons is special. Compared with unlabelled boutons TH-positive boutons had a higher proportion of their perimeter in contact with dendritic shafts and were more often in continuous contact with pairs of pre- and postsynaptic structures. However, this may result from exclusion from sites preferred by glutamatergic and GABAergic synapses as the density of all synapses in the closer vicinity was no different from any randomly selected site in the neuropil. This quantitative ultrastructural study presents basic features of the dopaminergic innervation in prefrontal area 10 and provides a more detailed understanding of the structural basis of dopamine signalling in the cortex.

Abstract

In common with other areas of the prefrontal cortex, activity in frontopolar area 10 is probably modulated by dopamine. We studied the dopaminergic innervation of monkey prefrontal area 10 by immunostaining with tyrosine hydroxylase (TH) antibodies. TH-positive axons in layer 3 were examined by electron microscopy of series of ultrathin sections. TH-positive boutons containing vesicles were sparse (2 × 10−4 per μm3) and the majority (94%, n = 52) had no identifiable synaptic specialization, which supports the hypothesis that dopamine is released non-synaptically and raises the question of whether the local microenvironment surrounding the boutons is special. Compared with unlabelled boutons TH-positive boutons had a higher proportion of their perimeter in contact with dendritic shafts and were more often in continuous contact with pairs of pre- and postsynaptic structures. However, this may result from exclusion from sites preferred by glutamatergic and GABAergic synapses as the density of all synapses in the closer vicinity was no different from any randomly selected site in the neuropil. This quantitative ultrastructural study presents basic features of the dopaminergic innervation in prefrontal area 10 and provides a more detailed understanding of the structural basis of dopamine signalling in the cortex.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Neuroinformatics
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2013
Deposited On:13 Feb 2014 13:07
Last Modified:08 Dec 2017 03:24
Publisher:Wiley-Blackwell
Number of Pages:11
ISSN:0953-816X
Publisher DOI:https://doi.org/10.1111/ejn.12124
PubMed ID:23331617

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