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Coherent diffraction and holographic imaging of individual biomolecules using low-energy electrons


Latychevskaia, Tatiana; Longchamp, Jean-Nicolas; Escher, Conrad; Fink, Hans-Werner (2013). Coherent diffraction and holographic imaging of individual biomolecules using low-energy electrons. In: Read, Randy; Urzhumtsev, Alexandre G; Lunin, Vladimir Y. Advancing Methods for Biomolecular Crystallography. Dordrecht: Springer, 331-342.

Abstract

Modern microscopy techniques are aimed at imaging an individual molecule at atomic resolution. Here we show that low-energy electrons with kinetic energies of 50–250 eV offer a possibility of overcome the problem of radiation damage, and obtaining images of individual biomolecules. Two experimental schemes for obtaining images of individual molecules – holography and coherent diffraction imaging – are discussed and compared. Images of individual molecules obtained by both techniques, using low-energy electrons, are shown.

Abstract

Modern microscopy techniques are aimed at imaging an individual molecule at atomic resolution. Here we show that low-energy electrons with kinetic energies of 50–250 eV offer a possibility of overcome the problem of radiation damage, and obtaining images of individual biomolecules. Two experimental schemes for obtaining images of individual molecules – holography and coherent diffraction imaging – are discussed and compared. Images of individual molecules obtained by both techniques, using low-energy electrons, are shown.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:07 Faculty of Science > Physics Institute
Dewey Decimal Classification:530 Physics
Language:English
Date:2013
Deposited On:27 Feb 2014 08:34
Last Modified:08 Dec 2017 04:33
Publisher:Springer
Series Name:NATO Science for Peace and Security Series A: Chemistry and Biology
ISBN:978-94-007-6231-2
Publisher DOI:https://doi.org/10.1007/978-94-007-6232-9_29

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