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Assessment of post-operative pain by nest complexity scoring in mice


Fleischmann, Thea. Assessment of post-operative pain by nest complexity scoring in mice. 2012, University of Zurich, Vetsuisse Faculty.

Abstract

Pain in laboratory mice is difficult to detect with conventional methods as mice do not obviously show symptoms of mild to moderate pain. Here we investigated the feasi-bility and reliability of nest building performance under various conditions as a meth-od to detect mild to moderate post-operative pain in laboratory mice and aimed to standardise this method for the routine use.
Female mice were randomly allocated into control (anaesthesia +/- analgesia) and surgery groups (minor laparotomy +/- analgesia) in two housing conditions. Animals were observed before (baseline) and after treatments (experiment). The nests were scored at seven time points with a numeric scoring system and latency of nest build-ing was measured as well as consumption of the nesting material.
Baseline nest scores were always higher than experimental scores and a reliable discrimination was found at three to nine hours after the start of the measurements. A clear graduation in nest complexity was seen after experiments, with higher nest scores in control groups than in surgery groups. Latency in the baseline was always shorter than in the experiment and latency in control groups was shorter than in sur-gery groups during experimental measurements. Pair housed mice had a slightly higher consumption of the nesting material than individually housed mice.
In conclusion, scoring of nest complexity at three to nine hours after laparotomy was useful to identify post-operative impairment, caused by moderate pain.

Abstract

Pain in laboratory mice is difficult to detect with conventional methods as mice do not obviously show symptoms of mild to moderate pain. Here we investigated the feasi-bility and reliability of nest building performance under various conditions as a meth-od to detect mild to moderate post-operative pain in laboratory mice and aimed to standardise this method for the routine use.
Female mice were randomly allocated into control (anaesthesia +/- analgesia) and surgery groups (minor laparotomy +/- analgesia) in two housing conditions. Animals were observed before (baseline) and after treatments (experiment). The nests were scored at seven time points with a numeric scoring system and latency of nest build-ing was measured as well as consumption of the nesting material.
Baseline nest scores were always higher than experimental scores and a reliable discrimination was found at three to nine hours after the start of the measurements. A clear graduation in nest complexity was seen after experiments, with higher nest scores in control groups than in surgery groups. Latency in the baseline was always shorter than in the experiment and latency in control groups was shorter than in sur-gery groups during experimental measurements. Pair housed mice had a slightly higher consumption of the nesting material than individually housed mice.
In conclusion, scoring of nest complexity at three to nine hours after laparotomy was useful to identify post-operative impairment, caused by moderate pain.

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Additional indexing

Item Type:Dissertation
Referees:Bürki Kurt, Graf Rolf
Communities & Collections:05 Vetsuisse Faculty > Institute of Laboratory Animal Science
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2012
Deposited On:29 Apr 2014 12:39
Last Modified:21 Nov 2017 20:52
Number of Pages:58

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