Publication: Microfluidic single-cell cultivation chip with controllable immobilization and selective release of yeast cells.
Microfluidic single-cell cultivation chip with controllable immobilization and selective release of yeast cells.
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Zhu, Z., Frey, O., Ottoz, D. S., Rudolf, F., & Hierlemann, A. (2012). Microfluidic single-cell cultivation chip with controllable immobilization and selective release of yeast cells. Lab on a Chip, 12(5), 906–915. https://doi.org/10.1039/c2lc20911j
Abstract
Abstract
Abstract
We present a microfluidic cell-culture chip that enables trapping, cultivation and release of selected individual cells. The chip is fabricated by a simple hybrid glass-SU-8-PDMS approach, which produces a completely transparent microfluidic system amenable to optical inspection. Single cells are trapped in a microfluidic channel using mild suction at defined cell immobilization orifices, where they are cultivated under controlled environmental conditions. Cells of interest can be individually and independently released for further do
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Creators (Authors)
- Zhu, Zhen
- Frey, Olivier
- Ottoz, Diana Silvia
- Rudolf, Fabian
- Hierlemann, Andreas
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Citations
Zhu, Z., Frey, O., Ottoz, D. S., Rudolf, F., & Hierlemann, A. (2012). Microfluidic single-cell cultivation chip with controllable immobilization and selective release of yeast cells. Lab on a Chip, 12(5), 906–915. https://doi.org/10.1039/c2lc20911j
