Phage Paride can kill dormant, antibiotic-tolerant cells of Pseudomonas aeruginosa by direct lytic replication

Maffei, Enea; Woischnig, Anne-Kathrin; Burkolter, Marco R; Heyer, Yannik; Humolli, Dorentina; Thürkauf, Nicole; Bock, Thomas; Schmidt, Alexander; Manfredi, Pablo; Egli, Adrian; Khanna, Nina; Jenal, Urs; Harms, Alexander

doi:10.1038/s41467-023-44157-3

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Maffei, E., Woischnig, A.-K., Burkolter, M. R., Heyer, Y., Humolli, D., Thürkauf, N., Bock, T., Schmidt, A., Manfredi, P., Egli, A., Khanna, N., Jenal, U., & Harms, A. (2024). Phage Paride can kill dormant, antibiotic-tolerant cells of Pseudomonas aeruginosa by direct lytic replication. Nature Communications, 15(1), 175. https://doi.org/10.1038/s41467-023-44157-3

Abstract

Bacteriophages are ubiquitous viral predators that have primarily been studied using fast-growing laboratory cultures of their bacterial hosts. However, microbial life in nature is mostly in a slow- or non-growing, dormant state. Here, we show that diverse phages can infect deep-dormant bacteria and suspend their replication until the host resuscitates ("hibernation"). However, a newly isolated Pseudomonas aeruginosa phage, named Paride, can directly replicate and induce the lysis of deep-dormant hosts. While non-growing bacteria are