Molecular dynamics analysis of the structural properties of the transglutaminases of Kutzneria albida and Streptomyces mobaraensis

Giordano, Deborah; Langini, Cassiano; Caflisch, Amedeo; Marabotti, Anna; Facchiano, Angelo

doi:10.1016/j.csbj.2022.07.024

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Giordano, D., Langini, C., Caflisch, A., Marabotti, A., & Facchiano, A. (2022). Molecular dynamics analysis of the structural properties of the transglutaminases of Kutzneria albida and Streptomyces mobaraensis. Computational and Structural Biotechnology Journal, 20, 3924–3934. https://doi.org/10.1016/j.csbj.2022.07.024

Abstract

The microbial transglutaminase (TGase) from Streptomyces mobaraensis (MTGase) is widely used for industrial applications. However, in the last decades, TGases from other bacteria have been described. We focused our attention on TGase, from Kutzneria albida (KalbTGase), recently characterized as more selective than MTGase and proposed for applications in drug delivery. By comparison of the crystallographic structures, the volume of the catalytic site results smaller in KalbTGase. We compared KalbTGase and MTGase structural flexibility