Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat

Johnson, P C D; Webster, L M I; Adam, A; Buckland, R; Dawson, D A; Keller, L F

doi:10.1016/j.molbiopara.2006.04.011

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Johnson, P. C. D., Webster, L. M. I., Adam, A., Buckland, R., Dawson, D. A., & Keller, L. F. (2006). Abundant variation in microsatellites of the parasitic nematode Trichostrongylus tenuis and linkage to a tandem repeat. Molecular and Biochemical Parasitology, 148(2), 210–218. https://doi.org/10.1016/j.molbiopara.2006.04.011

Abstract

An understanding of how genes move between and within populations of parasitic nematodes is important in combating the evolution and spread of anthelmintic, resistance. Much has been learned by studying mitochondrial DNA markers, but autosomal markers such as microsatellites have been applied to only a few nematode species, despite their many advantages for studying gene flow in eukaryotes. Here, we describe the isolation of 307 microsatellites from Trichostrongylus tenuis, an intestinal nematode of red grouse. High levels of variatio