The phagocytosis oxidase/Bem1p domain-containing protein PB1CP negatively regulates the NADPH oxidase RBOHD in plant immunity

Goto, Yukihisa; Maki, Noriko; Sklenar, Jan; Derbyshire, Paul; Menke, Frank L H; Zipfel, Cyril; Kadota, Yasuhiro; Shirasu, Ken

doi:10.1111/nph.19302

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Goto, Y., Maki, N., Sklenar, J., Derbyshire, P., Menke, F. L. H., Zipfel, C., Kadota, Y., & Shirasu, K. (2024). The phagocytosis oxidase/Bem1p domain-containing protein PB1CP negatively regulates the NADPH oxidase RBOHD in plant immunity. New Phytologist, 241(4), 1763–1779. https://doi.org/10.1111/nph.19302

Abstract

Perception of pathogen-associated molecular patterns (PAMPs) by surface-localized pattern recognition receptors activates RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) through direct phosphorylation by BOTRYTIS-INDUCED KINASE 1 (BIK1) and induces the production of reactive oxygen species (ROS). RBOHD activity must be tightly controlled to avoid the detrimental effects of ROS, but little is known about RBOHD downregulation. To understand the regulation of RBOHD, we used co-immunoprecipitation of RBOHD with mass spectrometry analysis and