Publication: PhotoTag: Photoactivatable Fluorophores for Protein Labeling
Date
Date
Date
2020
Journal Article
Published version
Abstract
Abstract
Abstract
We report experimental studies on the development of photoactivatable fluorophores for rapid, light-induced synthesis of protein conjugates. Proof-of-concept studies demonstrated that electronic excitation of photoactivatable BODIPY-ArN3 (1) in the presence of different proteins leads to efficient labeling in less than 10 min. After synthesis and isolation of the fluorescently tagged protein, photochemical conversion yields using human serum albumin and onartuzumab were 47 ± 7% and 42 ± 5%, respectively.
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4 since deposited on 2020-04-17
Acq. date: 2025-11-12
Views
1 since deposited on 2020-04-17
Acq. date: 2025-11-12
Additional indexing
Creators (Authors)
- Fay, Rachael
- Holland, Jason P
Volume
Volume
Volume
22
Number
Number
Number
9
Page range/Item number
Page range/Item number
Page range/Item number
3499
Page end
Page end
Page end
3503
Item Type
Item Type
Item Type
Journal Article
In collections
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Keywords
Physical and Theoretical Chemistry, Organic Chemistry, Biochemistry
Language
Language
Language
English
Publication date
Publication date
Publication date
2020-05-01
Date available
Date available
Date available
2020-04-17
ISSN or e-ISSN
ISSN or e-ISSN
ISSN or e-ISSN
1523-7052
OA Status
OA Status
OA Status
Green
Publisher DOI
Metrics
Downloads
4 since deposited on 2020-04-17
Acq. date: 2025-11-12
Views
1 since deposited on 2020-04-17
Acq. date: 2025-11-12
Green Open Access
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