Intra- and interlaboratory variability of allergen-specific IgE levels in atopic dogs in three different laboratories using the Fc-varepsilon receptor testing.

Thom, N; Favrot, C; Failing, K; Mueller, R S; Neiger, R; Linek, M

doi:10.5167/uzh-26334

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Thom, N., Favrot, C., Failing, K., Mueller, R. S., Neiger, R., & Linek, M. (2010). Intra- and interlaboratory variability of allergen-specific IgE levels in atopic dogs in three different laboratories using the Fc-varepsilon receptor testing. Veterinary Immunology and Immunopathology, 133, 183–189. https://doi.org/10.1016/j.vetimm.2009.07.019

Abstract

The IgE specific Fc-varepsilon receptor test is widely used for serological evaluation of allergic canine patients. Aim of the study was to evaluate intra- and interlaboratory variability in three independent laboratories. Duplicate serum aliquots from 15 atopic dogs were submitted simultaneously to 3 laboratories (LA, LB, LC), which subsequently performed the test in a blinded fashion. LA and LC analysed sera for 35 allergens and expressed the results as optical density units (OD), while LB analysed 15 allergens and provided reaction