Publication: MicroRNA expression studies: challenge of selecting reliable reference controls for data normalization
MicroRNA expression studies: challenge of selecting reliable reference controls for data normalization
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Madadi, S., Schwarzenbach, H., Lorenzen, J., & Soleimani, M. (2019). MicroRNA expression studies: challenge of selecting reliable reference controls for data normalization. Cellular and Molecular Life Sciences, 76(18), 3497–3514. https://doi.org/10.1007/s00018-019-03136-y
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Accurate determination of microRNA expression levels is a prerequisite in using these small non-coding RNA molecules as novel biomarkers in disease diagnosis and prognosis. Quantitative PCR is the method of choice for measuring the expression levels of microRNAs. However, a major obstacle that affects the reliability of results is the lack of validated reference controls for data normalization. Various non-coding RNAs have previously been used as reference controls, but their use may lead to variations and lack of comparability of mic
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- Madadi, Soheil
- Schwarzenbach, Heidi
- Lorenzen, Johan
- Soleimani, Meysam
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Madadi, S., Schwarzenbach, H., Lorenzen, J., & Soleimani, M. (2019). MicroRNA expression studies: challenge of selecting reliable reference controls for data normalization. Cellular and Molecular Life Sciences, 76(18), 3497–3514. https://doi.org/10.1007/s00018-019-03136-y