Sphk1 and Sphk2 Differentially Regulate Erythropoietin Synthesis in Mouse Renal Interstitial Fibroblast-like Cells

Hafizi, Redona; Imeri, Faik; Stepanovska Tanturovska, Bisera; Manaila, Roxana; Schwalm, Stephanie; Trautmann, Sandra; Wenger, Roland H; Pfeilschifter, Josef; Huwiler, Andrea

doi:10.3390/ijms23115882

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Hafizi, R., Imeri, F., Stepanovska Tanturovska, B., Manaila, R., Schwalm, S., Trautmann, S., Wenger, R. H., Pfeilschifter, J., & Huwiler, A. (2022). Sphk1 and Sphk2 Differentially Regulate Erythropoietin Synthesis in Mouse Renal Interstitial Fibroblast-like Cells. International Journal of Molecular Sciences, 23(11), 5882. https://doi.org/10.3390/ijms23115882

Abstract

Erythropoietin (Epo) is a crucial hormone regulating red blood cell number and consequently the hematocrit. Epo is mainly produced in the kidney by interstitial fibroblast-like cells. Previously, we have shown that in cultures of the immortalized mouse renal fibroblast-like cell line FAIK F3-5, sphingosine 1-phosphate (S1P), by activating S1P1 and S1P3 receptors, can stabilize hypoxia-inducible factor (HIF)-2α and upregulate Epo mRNA and protein synthesis. In this study, we have addressed the role of intracellular iS1P derived from sp