Evaluation of primer pairs for eDNA-based assessment of Ephemeroptera, Plecoptera, and Trichoptera across a biogeographically diverse region

Abstract

Macroinvertebrates serve as key indicators in ecological assessments of aquatic ecosystems, where the composition and richness of their communities are indicative of environmental and anthropogenic change. Established monitoring of macroinvertebrates is expensive and time-consuming, and relies on expert taxonomic knowledge. In contrast, biomonitoring based on molecular tools can support faster characterization of aquatic communities but needs validation for the target taxonomic groups and the study region. Here, we used data from a bi Show more