Publication: Single-Molecule Kinetic Studies of Nucleic Acids by Förster Resonance Energy Transfer
Single-Molecule Kinetic Studies of Nucleic Acids by Förster Resonance Energy Transfer
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Hadzic, M. C. A. S., Sigel, R. K. O., & Börner, R. (2022). Single-Molecule Kinetic Studies of Nucleic Acids by Förster Resonance Energy Transfer. In G. Steger, H. Rosenbach, & I. Span (Eds.), DNAzymes : Methods and Protocols (pp. 173–190). Springer. https://doi.org/10.1007/978-1-0716-2047-2_12
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Single-molecule microscopy is often used to observe and characterize the conformational dynamics of nucleic acids (NA). Due to the large variety of NA structures and the challenges specific to single-molecule observation techniques, the data recorded in such experiments must be processed via multiple statistical treatments to finally yield a reliable mechanistic view of the NA dynamics. In this chapter, we propose a comprehensive protocol to analyze single-molecule trajectories in the scope of single-molecule Förster resonance energy
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- Hadzic, Mélodie C A S
- Börner, Richard
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Hadzic, M. C. A. S., Sigel, R. K. O., & Börner, R. (2022). Single-Molecule Kinetic Studies of Nucleic Acids by Förster Resonance Energy Transfer. In G. Steger, H. Rosenbach, & I. Span (Eds.), DNAzymes : Methods and Protocols (pp. 173–190). Springer. https://doi.org/10.1007/978-1-0716-2047-2_12