Deep Learning-Assisted Nephrotoxicity Testing with Bioprinted Renal Spheroids

Tröndle, Kevin; Miotto, Guilherme; Rizzo, Ludovica; Pichler, Roman; Koch, Fritz; Koltay, Peter; Zengerle, Roland; Lienkamp, Soeren Sten; Kartmann, Sabrina; Zimmermann, Stefan

doi:10.18063/ijb.v8i2.528

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Tröndle, K., Miotto, G., Rizzo, L., Pichler, R., Koch, F., Koltay, P., Zengerle, R., Lienkamp, S. S., Kartmann, S., & Zimmermann, S. (2022). Deep Learning-Assisted Nephrotoxicity Testing with Bioprinted Renal Spheroids. International Journal of Bioprinting, 8(2), 528. https://doi.org/10.18063/ijb.v8i2.528

Abstract

We used arrays of bioprinted renal epithelial cell spheroids for toxicity testing with cisplatin. The concentration-dependent cell death rate was determined using a lactate dehydrogenase assay. Bioprinted spheroids showed enhanced sensitivity to the treatment in comparison to monolayers of the same cell type. The measured dose-response curves revealed an inhibitory concentration of the spheroids of IC ${50}$ = 9 ± 3 μM in contrast to the monolayers with IC ${50}$ = 17 ± 2 μM. Fluorescent labeling of a nephrotoxicity biomarker, kidne