Gene expression in human adipose-derived stem cells: comparison of 2D films, 3D electrospun meshes or co-cultured scaffolds with two-way paracrine effects

Hess, S C; Stark, W J; Mohn, D; Cohrs, N; Märsmann, S; Calcagni, M; Cinelli, P; Buschmann, J

doi:10.22203/eCM.v034a15

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Hess, S. C., Stark, W. J., Mohn, D., Cohrs, N., Märsmann, S., Calcagni, M., Cinelli, P., & Buschmann, J. (2017). Gene expression in human adipose-derived stem cells: comparison of 2D films, 3D electrospun meshes or co-cultured scaffolds with two-way paracrine effects. European Cells and Materials (ECM), 34, 232–248. https://doi.org/10.22203/eCM.v034a15

Abstract

Finding the appropriate cues to trigger the desired differentiation is a challenge in tissue engineering when stem cells are involved. In this regard, three-dimensional environments are often compared to cells' two-dimensional culture behaviour (plastic culture dish). Here, we compared the gene expression pattern of human adipose-derived stem cells (ASC) seeded in a three-dimensional (3D) electrospun mesh and on a two-dimensional (2D) film - both of exactly the same material. Additionally, we conducted experiments with a scaffold floa