Publication: Establishment of a coculture model for studying inflammation after pediatric cardiopulmonary bypass: from bench to bedside
Establishment of a coculture model for studying inflammation after pediatric cardiopulmonary bypass: from bench to bedside
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Wollersheim, S., Fedarava, K., Huebler, M., Schneiderhan-Marra, N., Berger, F., Miera, O., & Schmitt, K. R. (2012). Establishment of a coculture model for studying inflammation after pediatric cardiopulmonary bypass: from bench to bedside. Journal of Interferon & Cytokine Research, 32, 269–276. https://doi.org/10.1089/jir.2011.0096
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Cardiopulmonary bypass (CPB) has been known to induce an inflammatory response that is influenced by various factors. Hypothermia is supposed to reduce inflammation after CPB. We developed an in vitro coculture model for CPB and compared the effects of hypothermia on the inflammatory response in the coculture model with results from a clinical prospective randomized trial. The coculture model consisted of endothelial cells and monocytes. Cells were stimulated with tumor necrosis factor (TNF)-α and exposed to deep hypothermia (20°C) or
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- Wollersheim, Sonja
- Fedarava, Katisaryna
- Huebler, Michael
- Schneiderhan-Marra, Nicole
- Berger, Felix
- Miera, Oliver
- Schmitt, Katharina R
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Wollersheim, S., Fedarava, K., Huebler, M., Schneiderhan-Marra, N., Berger, F., Miera, O., & Schmitt, K. R. (2012). Establishment of a coculture model for studying inflammation after pediatric cardiopulmonary bypass: from bench to bedside. Journal of Interferon & Cytokine Research, 32, 269–276. https://doi.org/10.1089/jir.2011.0096
