Publication: Transcription and microbial profiling of body fluids using a massively parallel sequencing approach
Transcription and microbial profiling of body fluids using a massively parallel sequencing approach
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Salzmann, A. P., Russo, G., Aluri, S., & Haas, C. (2019). Transcription and microbial profiling of body fluids using a massively parallel sequencing approach. Forensic Science International. Genetics, 43, 102149. https://doi.org/10.1016/j.fsigen.2019.102149
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Analysis of biological evidence typically begins with a preliminary screening for the presence of biological fluids, traditionally with enzymatic or immunologic tests and of late by RNA profiling. The goal of this study was to create a whole transcriptome protocol, to view potential degradation effects in forensically relevant body fluids. Total RNA from fresh and aged blood, menstrual blood, saliva, semen, skin and vaginal secretion was analyzed with a massively parallel sequencing method. Two RNA-Seq library protocols with and witho
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Salzmann, A. P., Russo, G., Aluri, S., & Haas, C. (2019). Transcription and microbial profiling of body fluids using a massively parallel sequencing approach. Forensic Science International. Genetics, 43, 102149. https://doi.org/10.1016/j.fsigen.2019.102149