A MALDI-TOF MS library for rapid identification of human commensal gut bacteria from the class Clostridia

Asare, Paul Tetteh; Lee, Chi-Hsien; Hürlimann, Vera; Teo, Youzheng; Cuénod, Aline; Akduman, Nermin; Gekeler, Cordula; Afrizal, Afrizal; Corthesy, Myriam; Kohout, Claire; Thomas, Vincent; de Wouters, Tomas; Greub, Gilbert; Clavel, Thomas; Pamer, Eric G; Egli, Adrian; Maier, Lisa; Vonaesch, Pascale

doi:10.3389/fmicb.2023.1104707

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Asare, P. T., Lee, C.-H., Hürlimann, V., Teo, Y., Cuénod, A., Akduman, N., Gekeler, C., Afrizal, A., Corthesy, M., Kohout, C., Thomas, V., de Wouters, T., Greub, G., Clavel, T., Pamer, E. G., Egli, A., Maier, L., & Vonaesch, P. (2023). A MALDI-TOF MS library for rapid identification of human commensal gut bacteria from the class Clostridia. Frontiers in Microbiology, 14, 1104707. https://doi.org/10.3389/fmicb.2023.1104707

Abstract

INTRODUCTION: Microbial isolates from culture can be identified using 16S or whole-genome sequencing which generates substantial costs and requires time and expertise. Protein fingerprinting via Matrix-assisted Laser Desorption Ionization-time of flight mass spectrometry (MALDI-TOF MS) is widely used for rapid bacterial identification in routine diagnostics but shows a poor performance and resolution on commensal bacteria due to currently limited database entries. The aim of this study was to develop a MALDI-TOF MS plugin database (CL