3D microtissue–derived human stem cells seeded on electrospun nanocomposites under shear stress: Modulation of gene expression

Schneider, Isabelle; Baumgartner, Walter; Gröninger, Olivier; Stark, Wendelin J; Märsmann, Sonja; Calcagni, Maurizio; Cinelli, Paolo; Wolint, Petra; Buschmann, Johanna

doi:10.1016/j.jmbbm.2019.103481

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Schneider, I., Baumgartner, W., Gröninger, O., Stark, W. J., Märsmann, S., Calcagni, M., Cinelli, P., Wolint, P., & Buschmann, J. (2020). 3D microtissue–derived human stem cells seeded on electrospun nanocomposites under shear stress: Modulation of gene expression. Journal of the Mechanical Behavior of Biomedical Materials, 102, 103481. https://doi.org/10.1016/j.jmbbm.2019.103481

Abstract

Objective Different microenvironments trigger distinct differentiation of stem cells. Even without chemical supplementation, mechanical stimulation by shear stress may help to induce the desired differentiation. The cell format, such as three-dimensional (3D) microtissues (MTs), MT-derived cells or single cells (SCs), may have a pivotal impact as well. Here, we studied modulation of gene expression in human adipose–derived stem cells (ASCs) exposed to shear stress and/or after MT formation. Materials and methods Electrospun meshes of