Publication: Exosomes as Carriers for Notch Molecules
Exosomes as Carriers for Notch Molecules
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Marconi, G. D., Diomede, F., Trubiani, O., Porcheri, C., & Mitsiadis, T. A. (2022). Exosomes as Carriers for Notch Molecules. In J. Dongyu (Ed.), Notch Signaling Research : Methods and Protocols (No. 2472; pp. 197–208). Springer. https://doi.org/10.1007/978-1-0716-2201-8_16
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Exosomes are extracellular vesicles involved in cell-to-cell communication as well as extrusion of biological material. Using dental pulp stem cells culture as a model, we hereby describe a method for the packaging of Delta-like 4 (DLL4), a representative Notch ligand, into newly generated exosomes. We then provide methods of analysis to confirm the presence of Notch proteins and transcripts internalization and transport via exosomes.
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Marconi, G. D., Diomede, F., Trubiani, O., Porcheri, C., & Mitsiadis, T. A. (2022). Exosomes as Carriers for Notch Molecules. In J. Dongyu (Ed.), Notch Signaling Research : Methods and Protocols (No. 2472; pp. 197–208). Springer. https://doi.org/10.1007/978-1-0716-2201-8_16