Publication: Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics
Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics
Date
Date
Date
Citations
Hunziker, A., & Stertz, S. (2022). Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics. STAR Protocols, 3(1), 101089. https://doi.org/10.1016/j.xpro.2021.101089
Abstract
Abstract
Abstract
Due to the low stoichiometry and highly transient nature of protein phosphorylation it is challenging to capture the dynamics and complexity of phosphorylation events on a systems level. Here, we present an optimized protocol to measure virus-induced phosphorylation events with high sensitivity using label free quantification-based phosphoproteomics. Specifically, we describe filter assisted protein digestion (FASP), enrichment of phosphopeptides, mass spectrometry, and subsequent bioinformatic analysis. For complete details on the us
Additional indexing
Creators (Authors)
- Hunziker, Annika
- Stertz, Silke
Volume
Volume
Volume
Number
Number
Number
Page range/Item number
Page range/Item number
Page range/Item number
Item Type
Item Type
Item Type
In collections
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Language
Language
Language
Publication date
Publication date
Publication date
Date available
Date available
Date available
ISSN or e-ISSN
ISSN or e-ISSN
ISSN or e-ISSN
Additional Information
Additional Information
Additional Information
OA Status
OA Status
OA Status
Free Access at
Free Access at
Free Access at
Publisher DOI
Citations
Hunziker, A., & Stertz, S. (2022). Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics. STAR Protocols, 3(1), 101089. https://doi.org/10.1016/j.xpro.2021.101089
