Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

de Medeiros, Gustavo; Kromm, Dimitri; Balazs, Balint; Norlin, Nils; Günther, Stefan; Izquierdo, Emiliano; Ronchi, Paolo; Komoto, Shinya; Krzic, Uros; Schwab, Yannick; Peri, Francesca; de Renzis, Stefano; Leptin, Maria; Rauzi, Matteo; Hufnagel, Lars

doi:10.1038/s41598-019-54349-x

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de Medeiros, G., Kromm, D., Balazs, B., Norlin, N., Günther, S., Izquierdo, E., Ronchi, P., Komoto, S., Krzic, U., Schwab, Y., Peri, F., de Renzis, S., Leptin, M., Rauzi, M., & Hufnagel, L. (2020). Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Scientific Reports, 10, 1942. https://doi.org/10.1038/s41598-019-54349-x

Abstract

Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub