Publication:

Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

Date

Date

Date
2020
Journal Article
Published version
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cris.lastimport.scopus2025-06-02T03:43:53Z
cris.lastimport.wos2025-07-22T01:31:28Z
dc.contributor.institutionUniversity of Zurich
dc.date.accessioned2020-02-20T13:13:45Z
dc.date.available2020-02-20T13:13:45Z
dc.date.issued2020-12-01
dc.description.abstract

Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.

dc.identifier.doi10.1038/s41598-019-54349-x
dc.identifier.issn2045-2322
dc.identifier.scopus2-s2.0-85079063271
dc.identifier.urihttps://www.zora.uzh.ch/handle/20.500.14742/168241
dc.identifier.wos000559758600005
dc.language.isoeng
dc.subjectMultidisciplinary
dc.subject.ddc570 Life sciences; biology
dc.title

Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

dc.typearticle
dcterms.accessRightsinfo:eu-repo/semantics/openAccess
dcterms.bibliographicCitation.journaltitleScientific Reports
dcterms.bibliographicCitation.originalpublishernameNature Publishing Group
dcterms.bibliographicCitation.pagestart1942
dcterms.bibliographicCitation.volume10
dspace.entity.typePublicationen
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg, Friedrich Miescher Institute for Biomedical Research
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg, Universität Heidelberg
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg, Luxendo GmbH
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg, Okinawa Institute of Science and Technology Graduate University
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg, University of Zurich
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg, Université Côte d'Azur
uzh.contributor.affiliationEuropean Molecular Biology Laboratory Heidelberg
uzh.contributor.authorde Medeiros, Gustavo
uzh.contributor.authorKromm, Dimitri
uzh.contributor.authorBalazs, Balint
uzh.contributor.authorNorlin, Nils
uzh.contributor.authorGünther, Stefan
uzh.contributor.authorIzquierdo, Emiliano
uzh.contributor.authorRonchi, Paolo
uzh.contributor.authorKomoto, Shinya
uzh.contributor.authorKrzic, Uros
uzh.contributor.authorSchwab, Yannick
uzh.contributor.authorPeri, Francesca
uzh.contributor.authorde Renzis, Stefano
uzh.contributor.authorLeptin, Maria
uzh.contributor.authorRauzi, Matteo
uzh.contributor.authorHufnagel, Lars
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceYes
uzh.contributor.correspondenceNo
uzh.document.availabilitypublished_version
uzh.eprint.datestamp2020-02-20 13:13:45
uzh.eprint.lastmod2025-07-22 01:36:54
uzh.eprint.statusChange2020-02-20 13:13:45
uzh.harvester.ethYes
uzh.harvester.nbNo
uzh.identifier.doi10.5167/uzh-184734
uzh.jdb.eprintsId18727
uzh.oastatus.unpaywallgold
uzh.oastatus.zoraGold
uzh.publication.citationde Medeiros, Gustavo; Kromm, Dimitri; Balazs, Balint; Norlin, Nils; Günther, Stefan; Izquierdo, Emiliano; Ronchi, Paolo; Komoto, Shinya; Krzic, Uros; Schwab, Yannick; Peri, Francesca; de Renzis, Stefano; Leptin, Maria; Rauzi, Matteo; Hufnagel, Lars (2020). Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Scientific Reports, 10:1942.
uzh.publication.freeAccessAtdoi
uzh.publication.originalworkoriginal
uzh.publication.publishedStatusfinal
uzh.scopus.impact24
uzh.scopus.subjectsMultidisciplinary
uzh.workflow.doajuzh.workflow.doaj.true
uzh.workflow.eprintid184734
uzh.workflow.fulltextStatuspublic
uzh.workflow.revisions42
uzh.workflow.rightsCheckkeininfo
uzh.workflow.sourceCrossRef:10.1038/s41598-019-54349-x
uzh.workflow.statusarchive
uzh.wos.impact21
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