Changes in $Medicago$ $truncatula$ seed proteome along the rehydration–dehydration cycle highlight new players in the genotoxic stress response

Pagano, Andrea; Kunz, Laura; Dittmann, Antje; Araújo, Susana De Sousa; Macovei, Anca; Shridhar Gaonkar, Shraddha; Sincinelli, Federico; Wazeer, Hisham; Balestrazzi, Alma

doi:10.3389/fpls.2023.1188546

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Pagano, A., Kunz, L., Dittmann, A., Araújo, S. D. S., Macovei, A., Shridhar Gaonkar, S., Sincinelli, F., Wazeer, H., & Balestrazzi, A. (2023). Changes in $Medicago$ $truncatula$ seed proteome along the rehydration–dehydration cycle highlight new players in the genotoxic stress response. Frontiers in Plant Science, 14, 1188546. https://doi.org/10.3389/fpls.2023.1188546

Abstract

IntroductionSeveral molecular aspects underlying the seed response to priming and the resulting vigor profile are still poorly understood. Mechanisms involved in genome maintenance deserve attention since the balance between stimulation of germination and DNA damage accumulation versus active repair is a key determinant for designing successful seed priming protocols.MethodsChanges in the Medicago truncatula seed proteome were investigated in this study, using discovery mass spectrometry and label-free quantification, along the rehydr