Publication: Direct observation of selective protein capturing on molecular imprinting substrates
Direct observation of selective protein capturing on molecular imprinting substrates
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Fukazawa, K., Li, Q., Seeger, S., & Ishihara, K. (2013). Direct observation of selective protein capturing on molecular imprinting substrates. Biosensors & Bioelectronics, 40(1), 96–101. https://doi.org/10.1016/j.bios.2012.06.033
Abstract
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Abstract
A sensing interface for specific protein capture was fabricated using a novel molecular imprinting (MIP) process. Bovine serum albumin (BSA) and ovalbumin (OVA) were imprinted on a quartz substrate with modified alkyl groups, and target protein capture was detected using a deep-UV fluorescence image microscope (UVFLIM). The imprinted protein was immobilized to silica beads (diameter: 15 μm) using a phospholipid polymer containing both active ester groups and silane coupling groups, which were used as protein stamps to prepare the impr
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- Fukazawa, Kyoko
- Li, Qiang
- Seeger, Stefan
- Ishihara, Kazuhiko
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Fukazawa, K., Li, Q., Seeger, S., & Ishihara, K. (2013). Direct observation of selective protein capturing on molecular imprinting substrates. Biosensors & Bioelectronics, 40(1), 96–101. https://doi.org/10.1016/j.bios.2012.06.033