Insulin induces steroidogenesis in canine luteal cells via PI3K-MEK-MAPK

Silva, Renata Santos; Mattoso Miskulin Cardoso, Ana Paula; Giometti, Ines Cristina; D'Aprile, Loren; Garcia Santos, Francislaine Anelize; Maruyama, Arnaldo Shindi; Medeiros de Carvalho Sousa, Liza Margareth; Unniappan, Suraj; Kowalewski, Mariusz P; de Carvalho Papa, Paula

doi:10.1016/j.mce.2021.111518

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Silva, R. S., Mattoso Miskulin Cardoso, A. P., Giometti, I. C., D’Aprile, L., Garcia Santos, F. A., Maruyama, A. S., Medeiros de Carvalho Sousa, L. M., Unniappan, S., Kowalewski, M. P., & de Carvalho Papa, P. (2022). Insulin induces steroidogenesis in canine luteal cells via PI3K-MEK-MAPK. Molecular and Cellular Endocrinology, 540, 111518. https://doi.org/10.1016/j.mce.2021.111518

Abstract

Glucose uptake increases in canine luteal cells under insulin treatment. We hypothesize that insulin also increases luteal cell steroidogenesis. Dogs underwent elective ovariohysterectomy from days 10-60 post ovulation and their corpora lutea (CL) and blood samples were collected. Deep RNA sequencing determined differentially expressed genes in CL; those related to insulin signaling and steroidogenesis were validated in vivo by qPCR and their respective proteins by Western blotting and immunofluorescence. Next, luteal cell cultures we