Publication: Development and application of a multiplex TaqMan® real-time qPCR assay for the simultaneous detection of Anaplasma marginale and Theileria annulata and molecular characterization of Anaplasma marginale from cattle in Western Cuba
Development and application of a multiplex TaqMan® real-time qPCR assay for the simultaneous detection of Anaplasma marginale and Theileria annulata and molecular characterization of Anaplasma marginale from cattle in Western Cuba
Date
Date
Date
Citations
Díaz-Sánchez, A. A., Meli, M. L., Obregón Álvarez, D., Fonseca-Rodríguez, O., Cabezas-Cruz, A., Hofmann-Lehmann, R., & Corona-González, B. (2020). Development and application of a multiplex TaqMan® real-time qPCR assay for the simultaneous detection of Anaplasma marginale and Theileria annulata and molecular characterization of Anaplasma marginale from cattle in Western Cuba. Ticks and Tick-Borne Diseases, 11(2), 101356. https://doi.org/10.1016/j.ttbdis.2019.101356
Abstract
Abstract
Abstract
Anaplasmosis and theileriosis are considered the most important tick-borne diseases for livestock production worldwide, causing significant economic losses in tropical and subtropical regions. The present study was aimed to develop a multiplex TaqMan® qPCR assay to simultaneously detect Anaplasma marginale and Theileria annulata and to applied it to investigate naturally infected cattle in Cuba. The assay was highly specific, sensible, and efficient; it was more sensitive than a well-established nested PCR and detected 1 DNA copy of e
Metrics
Downloads
Views
Additional indexing
Creators (Authors)
- Díaz-Sánchez, Adrian Alberto
- Meli, Marina L
- Obregón Álvarez, Dasiel
- Fonseca-Rodríguez, Osvaldo
- Cabezas-Cruz, Alejandro
- Corona-González, Belkis
Volume
Volume
Volume
Number
Number
Number
Page range/Item number
Page range/Item number
Page range/Item number
Item Type
Item Type
Item Type
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Keywords
Language
Language
Language
Publication date
Publication date
Publication date
Date available
Date available
Date available
ISSN or e-ISSN
ISSN or e-ISSN
ISSN or e-ISSN
OA Status
OA Status
OA Status
Publisher DOI
Metrics
Downloads
Views
Citations
Díaz-Sánchez, A. A., Meli, M. L., Obregón Álvarez, D., Fonseca-Rodríguez, O., Cabezas-Cruz, A., Hofmann-Lehmann, R., & Corona-González, B. (2020). Development and application of a multiplex TaqMan® real-time qPCR assay for the simultaneous detection of Anaplasma marginale and Theileria annulata and molecular characterization of Anaplasma marginale from cattle in Western Cuba. Ticks and Tick-Borne Diseases, 11(2), 101356. https://doi.org/10.1016/j.ttbdis.2019.101356
