Publication: Doa10/MARCH6 architecture interconnects E3 ligase activity with lipid-binding transmembrane channel to regulate SQLE
Date
Date
Date
2024
Journal Article
Published version
Abstract
Abstract
Abstract
Transmembrane E3 ligases play crucial roles in homeostasis. Much protein and organelle quality control, and metabolic regulation, are determined by ER-resident MARCH6 E3 ligases, including Doa10 in yeast. Here, we present Doa10/MARCH6 structural analysis by cryo-EM and AlphaFold predictions, and a structure-based mutagenesis campaign. The majority of Doa10/MARCH6 adopts a unique circular structure within the membrane. This channel is established by a lipid-binding scaffold, and gated by a flexible helical bundle. The ubiquitylation ac
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9 since deposited on 2024-01-31
Acq. date: 2025-11-12
Views
2 since deposited on 2024-01-31
Acq. date: 2025-11-12
Additional indexing
Creators (Authors)
- Botsch, J Josephine
- Junker, Roswitha
- Sorgenfrei, Michèle
- Ogger, Patricia P
- Stier, Luca
- von Gronau, Susanne
- Murray, Peter J
- Seeger, Markus A
- Schulman, Brenda A
- Bräuning, Bastian
Volume
Volume
Volume
15
Number
Number
Number
1
Page range/Item number
Page range/Item number
Page range/Item number
410
Item Type
Item Type
Item Type
Journal Article
In collections
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Language
Language
Language
English
Publication date
Publication date
Publication date
2024-01-09
Date available
Date available
Date available
2024-01-31
ISSN or e-ISSN
ISSN or e-ISSN
ISSN or e-ISSN
2041-1723
OA Status
OA Status
OA Status
Gold
Free Access at
Free Access at
Free Access at
DOI
Publisher DOI
Metrics
Downloads
9 since deposited on 2024-01-31
Acq. date: 2025-11-12
Views
2 since deposited on 2024-01-31
Acq. date: 2025-11-12
Gold Open Access
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