Inhibition of herpes simplex virus type 1 replication by Adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/Helicase activities

Glauser, D L; Seyffert, M; Strasser, R; Franchini, M; Laimbacher, A S; Dresch, C; de Oliveirea, A P; Vogel, R; Büning, H; Salvetti, A; Ackermann, M; Fraefel, C

doi:10.1128/JVI.01503-09

Citations

Citation copied

Glauser, D. L., Seyffert, M., Strasser, R., Franchini, M., Laimbacher, A. S., Dresch, C., de Oliveirea, A. P., Vogel, R., Büning, H., Salvetti, A., Ackermann, M., & Fraefel, C. (2010). Inhibition of herpes simplex virus type 1 replication by Adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/Helicase activities. Journal of Virology, 84(8), 3808–3824. https://doi.org/10.1128/JVI.01503-09

Abstract

Adeno-associated virus (AAV) has previously been shown to inhibit the replication of its helper virus herpes simplex virus type 1 (HSV-1), and the inhibitory activity has been attributed to the expression of the AAV Rep proteins. In the present study, we assessed the Rep activities required for inhibition of HSV-1 replication using a panel of wild-type and mutant Rep proteins lacking defined domains and activities. We found that the inhibition of HSV-1 replication required Rep DNA-binding and ATPase/helicase activities but not endonuc