A primary culture of distal convoluted tubules expressing functional thiazide-sensitive NaCl transport

Markadieu, Nicolas; San-Cristobal, Pedro; Nair, Anil V; Verkaart, Sjoerd; Lenssen, Ellen; Tudpor, Kukiat; van Zeeland, Femke; Loffing, Johannes; Bindels, René J M

doi:10.1152/ajprenal.00114.2012

Citations

Citation copied

Markadieu, N., San-Cristobal, P., Nair, A. V., Verkaart, S., Lenssen, E., Tudpor, K., van Zeeland, F., Loffing, J., & Bindels, R. J. M. (2012). A primary culture of distal convoluted tubules expressing functional thiazide-sensitive NaCl transport. American Journal of Physiology : Renal Physiology, F886–F892. https://doi.org/10.1152/ajprenal.00114.2012

Abstract

Studying the molecular regulation of the thiazide-sensitive Na(+)-Cl(-) cotransporter (NCC) is important for understanding how the kidney contributes to blood pressure regulation. Until now, a native mammalian cell model to investigate this transporter remained unknown. Our aim here is to establish, for the first time, a primary distal convoluted tubule (DCT) cell culture exhibiting transcellular thiazide-sensitive Na(+) transport. Because parvalbumin (PV) is primarily expressed in the DCT, where it colocalizes with NCC, kidneys from