Publication: Quantitative and dynamic assay of single cell chemotaxis
Quantitative and dynamic assay of single cell chemotaxis
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Lee, S. S., Horvath, P., Pelet, S., Hegemann, B., Lee, L. P., & Peter, M. (2012). Quantitative and dynamic assay of single cell chemotaxis. Integrative Biology, 4(4), 381. https://doi.org/10.1039/c2ib00144f
Abstract
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Abstract
We have developed a single-cell assay platform that allows quantitative analysis of single cell chemotaxis by dynamic morphogenetic gradients, subcellular microscopic imaging and automated image analysis, and have applied these to measure cellular polarization of budding yeast. The computer-controlled microfluidic device regulates the gradient profile at any given time, and allows quantitative monitoring of cell morphology and the localization and expression of specific marker proteins during the dynamic polarization process. With thi
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- Lee, Sung Sik
- Horvath, Peter
- Pelet, Serge
- Hegemann, Björn
- Lee, Luke P
- Peter, Matthias
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Lee, S. S., Horvath, P., Pelet, S., Hegemann, B., Lee, L. P., & Peter, M. (2012). Quantitative and dynamic assay of single cell chemotaxis. Integrative Biology, 4(4), 381. https://doi.org/10.1039/c2ib00144f
