Publication: 3D restoration microscopy improves quantification of enzyme-labeled fluorescence-based single-cell phosphatase activity in plankton
3D restoration microscopy improves quantification of enzyme-labeled fluorescence-based single-cell phosphatase activity in plankton
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Diaz-de-Quijano, D., Palacios, P., Horňák, K., & Felip, M. (2014). 3D restoration microscopy improves quantification of enzyme-labeled fluorescence-based single-cell phosphatase activity in plankton. Cytometry. Part A, 85(10), 841–853. https://doi.org/10.1002/cyto.a.22486
Abstract
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Abstract
The ELF or fluorescence-labeled enzyme activity (FLEA) technique is a culture-independent single-cell tool for assessing plankton enzyme activity in close-to-in situ conditions. We demonstrate that single-cell FLEA quantifications based on two-dimensional (2D) image analysis were biased by up to one order of magnitude relative to deconvolved 3D. This was basically attributed to out-of-focus light, and partially to object size. Nevertheless, if sufficient cells were measured (25-40 cells), biases in individual 2D cell measurements were
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- Diaz-de-Quijano, Daniel
- Palacios, Pilar
- Horňák, Karel
- Felip, Marisol
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Citations
Diaz-de-Quijano, D., Palacios, P., Horňák, K., & Felip, M. (2014). 3D restoration microscopy improves quantification of enzyme-labeled fluorescence-based single-cell phosphatase activity in plankton. Cytometry. Part A, 85(10), 841–853. https://doi.org/10.1002/cyto.a.22486
