Publication: Long-term C. elegans immobilization enables high resolution developmental studies in vivo
Long-term C. elegans immobilization enables high resolution developmental studies in vivo
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Berger, S., Lattmann, E., Aegerter-Wilmsen, T., Hengartner, M., Hajnal, A., deMello, A., & Casadevall i Solvas, X. (2018). Long-term C. elegans immobilization enables high resolution developmental studies in vivo. Lab on a Chip, 18(9), 1359–1368. https://doi.org/10.1039/c7lc01185g
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Live-imaging of C. elegans is essential for the study of conserved cellular pathways (e.g. EGFR/Wnt signaling) and morphogenesis in vivo. However, the usefulness of live imaging as a research tool has been severely limited by the need to immobilize worms prior to and during imaging. Conventionally, immobilization is achieved by employing both physical and chemical interventions. These are known to significantly affect many physiological processes, and thus limit our understanding of dynamic developmental processes. Herein we present a
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- Berger, Simon
- Lattmann, Evelyn
- Aegerter-Wilmsen, Tinri
- Hengartner, Michael
- deMello, Andrew
- Casadevall i Solvas, Xavier
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Berger, S., Lattmann, E., Aegerter-Wilmsen, T., Hengartner, M., Hajnal, A., deMello, A., & Casadevall i Solvas, X. (2018). Long-term C. elegans immobilization enables high resolution developmental studies in vivo. Lab on a Chip, 18(9), 1359–1368. https://doi.org/10.1039/c7lc01185g