Extracellular Vesicles from 3D Engineered Microtissues Harbor Disease-Related Cargo Absent in EVs from 2D Cultures

Millan, Christopher; Prause, Lukas Werner; Vallmajo-Martin, Queralt; Hensky, Natalie; Eberli, Daniel

doi:10.1002/adhm.202002067

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Millan, C., Prause, L. W., Vallmajo-Martin, Q., Hensky, N., & Eberli, D. (2022). Extracellular Vesicles from 3D Engineered Microtissues Harbor Disease-Related Cargo Absent in EVs from 2D Cultures. Advanced Healthcare Materials, 11(5), 2002067. https://doi.org/10.1002/adhm.202002067

Abstract

Engineered microtissues that recapitulate key properties of the tumor microenvironment can induce clinically relevant cancer phenotypes in vitro. However, their effect on molecular cargo of secreted extracellular vesicles (EVs) has not yet been investigated. Here, the impact of hydrogel-based 3D engineered microtissues on EVs secreted by benign and malignant prostate cells is assessed. Compared to 2D cultures, yield of EVs per cell is significantly increased for cancer cells cultured in 3D. Whole transcriptome sequencing and proteomic