Publication: Ligand Binding Studied by 2D IR Spectroscopy Using the Azidohomoalanine Label
Ligand Binding Studied by 2D IR Spectroscopy Using the Azidohomoalanine Label
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Bloem, R., Koziol, K., Waldauer, S. A., Buchli, B., Walser, R., Samatanga, B., Jelesarov, I., & Hamm, P. (2012). Ligand Binding Studied by 2D IR Spectroscopy Using the Azidohomoalanine Label. Journal of Physical Chemistry B, 116(46), 13705–13712. https://doi.org/10.1021/jp3095209
Abstract
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Abstract
We explore the capability of the azidohomoalanine (Aha) as a vibrational label for 2D IR spectroscopy to study the binding of the target peptide to the PDZ2 domain. The Aha label responds sensitively to its local environment and its peak extinction coefficient of 350-400 M(-1) cm(-1) is high enough to routinely measure it in the low millimolar concentration regime. The central frequency, inhomogeneous width and spectral diffusion times deduced from the 2D IR line shapes of the Aha label at various positions in the peptide sequence is
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- Bloem, Robbert
- Koziol, Klemens
- Waldauer, Steven A
- Buchli, Brigitte
- Walser, Reto
- Samatanga, Brighton
- Jelesarov, Ilian
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Bloem, R., Koziol, K., Waldauer, S. A., Buchli, B., Walser, R., Samatanga, B., Jelesarov, I., & Hamm, P. (2012). Ligand Binding Studied by 2D IR Spectroscopy Using the Azidohomoalanine Label. Journal of Physical Chemistry B, 116(46), 13705–13712. https://doi.org/10.1021/jp3095209
